![]() As amostras foram extraídas com metanol: água (1:1) em banho de ultra-som. A linearidade, precisão, recuperação, limites de detecção e quantificação e seletividade foram os parâmetros de desempenho avaliados. No presente trabalho, um método utilizando cromatografia líquida de alta eficiência foi otimizado e validado para quantificar o LSD em selos apreendidos em Minas Gerais. High Performance Liquid Chromatography/quantitative analysis. Thus, the method used showed satisfactory analytical performance, and proved suitable as an analytical tool for LSD determination in illicit samples seized by police forces. The samples of blotters (n =22) were analyzed and the mean value of 67.55 μg of LSD/blotter (RSD=27.5%) was found. The limits of detection and quantification were 0.01 and 0.05 μg/mL, respectively (0.1 and 0.5 μg of LSD/blotter). The linearity between 0.05 and 20.00 μg/mL (0.5 and 200.0μg of LSD/blotter) was observed with satisfactory mean intra and inter assay precision (RSD r = 4.4% and RSD R = 6.4%, respectively) and with mean recoveries of 83.4% and 84.9% to the levels of 1.00 and 20.00 μg/mL (10 and 200μg LSD/blotter). The samples were extracted with methanol:water (1: 1) in an ultra-sound bath. Linearity, precision, recovery, limits of detection and quantification, and selectivity were the parameters used to evaluate performance. In the present study, a method using high performance liquid chromatography to quantify LSD, in blotter papers seized in Minas Gerais, was optimized and validated. ![]() IIToxicology Laboratory, Pharmacy College, Federal University of Minas Gerais IInstitute of Criminology of Minas Gerais, Belo Horizonte Rua Juiz de Fora, 400 - Bairro Barro Preto - 30180-060 - Belo Horizonte - MG, Brasil. Instituto de Criminalística de Minas Gerais. Quantification of LSD in illicit samples by high performance liquid chromatographyĬorrespondence: P. LSD Química Forense Toxicologia analítica Validação intralaboratorial Cromatografia líquida de alta efeciência Desta forma, o método analítico apresentou desempenho satisfatório, capaz de ser utilizado como instrumento de análise para a determinação do LSD em amostras ilícitas apreendidas pelas forças policiais. As amostras de selos (n = 22) foram analisadas e o valor médio encontrado foi de 67,55 μg de LSD/selo (RSD% = 27,5). Os limites de detecção e quantificação encontrados foram de 0,01 e 0,05 mg/mL, respectivamente (0,1 e 0,5 μg LSD/selo). A linearidade entre 0,05 a 20,00 mg/mL (0,5 a 200 μg LSD/blotter) foi observada com precisão média, intra e inter ensaio, satisfatória (RSDr = 4,4% e RSD R = 6,4%, respectivamente) e com recuperações médias de 83,4% e 84,9% para os níveis de LSD de 1,00 e 20,00 mg/mL (10 e 200 μg LSD/selo). ![]() LSD Forensic chemistry Analytical toxicology In-house validation High Performance Liquid Chromatography ![]() The linearity between 0.05 and 20.00 μg/mL (0.5 and 200.0μg of LSD/blotter) was observed with satisfactory mean intra and inter assay precision (RSDr = 4.4% and RSD R = 6.4%, respectively) and with mean recoveries of 83.4% and 84.9% to the levels of 1.00 and 20.00 μg/mL (10 and 200μg LSD/blotter).
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